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Functional characterization of a thermostable methionine adenosyltransferase from
Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan
《化学科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 238-244 doi: 10.1007/s11705-016-1566-2
关键词: ion-preference methionine adenosyltransferase secondary structure thermostability Thermus thermophilus
《环境科学与工程前沿(英文)》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1368-0
• SMX was mainly degraded by hydrolysis, isoxazole oxidation and double-bond addition.
关键词: Sulfamethoxazole Ozonation Natural organic matters Secondary effluent Degradation mechanism
Physical and chemical processes of wintertime secondary nitrate aerosol formation
Qi YING
《环境科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 348-361 doi: 10.1007/s11783-011-0343-1
关键词: secondary nitrate aerosol N2O5 heterogeneous reaction process analysis
Two-echelon Emergency Response Problem and Simulation Considering Secondary Disasters
Han-peng Zhang,Yi Liao,Hui-xia Luo
《工程管理前沿(英文)》 2014年 第1卷 第3期 页码 318-321 doi: 10.15302/J-FEM-2014042
关键词: simulation secondary disasters two-echelon vehicle routing model
Formation of secondary inorganic aerosol in a frigid urban atmosphere
《环境科学与工程前沿(英文)》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1452-0
•Harbin showed relatively high threshold RH (80%) for apparent increase of SOR.
关键词: Haze Sulfate Nitrate Heterogeneous chemistry Biomass burning Northeast China
Haiqian LI, Yonglong LU, Li LI
《环境科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 589-597 doi: 10.1007/s11783-013-0495-2
关键词:
polychlorinated dibenzo-
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 137-146 doi: 10.1007/s11684-016-0486-3
This study aims to elucidate the underlying molecular mechanisms of artemisinin accumulation induced by cadmium (Cd). The effects of different Cd concentrations (0, 20, 60, and 120 μmol/L) on the biosynthesis of Artemisia annua L. were examined. Intermediate and end products were quantified by HPLC-ESI-MS/MS analysis. The expression of key biosynthesis enzymes was also determined by qRT-PCR. The results showed that the application of treatment with 60 and 120 μmol/L Cd for 3 days significantly improved the biosynthesis of artemisinic acid, arteannuin B, and artemisinin. The concentrations of artemisinic acid, arteannuin B, and artemisinin in the 120 μmol/L Cd-treated group were 2.26, 102.08, and 33.63 times higher than those in the control group, respectively. The concentrations of arteannuin B and artemisinin in 60 μmol/L Cd-treated leaves were 61.10 and 26.40 times higher than those in the control group, respectively. The relative expression levels of HMGR, FPS, ADS, CYP71AV1, DBR2, ALDH1, and DXR were up-regulated in the 120 μmol/L Cd-treated group because of increased contents of artemisinic metabolites after 3 days of treatment. Hence, appropriate doses of Cd can increase the concentrations of artemisinic metabolites at a certain time point by up-regulating the relative expression levels of key enzyme genes involved in artemisinin biosynthesis.
关键词: Cd secondary metabolites gene expressions Artemisia annua L.
Simple model of sludge thickening process in secondary settlers
Yuankai ZHANG,Hongchen WANG,Lu QI,Guohua LIU,Zhijiang HE,Songzhu JIANG
《环境科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 319-326 doi: 10.1007/s11783-014-0758-6
关键词: wastewater treatment plants secondary settler sludge thickening sludge settling hindered zone
On the location of the secondary instantaneous poles in two-degree-of-freedom spherical mechanisms
Soheil ZARKANDI
《机械工程前沿(英文)》 2014年 第9卷 第1期 页码 34-40 doi: 10.1007/s11465-014-0290-1
As the instant centers in planar mechanisms, the instantaneous poles (or instant poles, in brief) can be used for kinematic analysis in spherical mechanisms. One of the mandatory steps in this analysis is the determination of the location of these poles. This paper presents a theorem showing analytically that the locus of an unknown secondary instant pole in two-degree-of-freedom (2-DOF) spherical mechanisms is a great circle (GC). The exact location of the pole on its GC is obtained based on the configuration of the mechanism and velocity ratio of the two inputs. Moreover, using the results of the theorem, a geometrical technique is presented to determine the GC of the pole.
关键词: spherical mechanisms instantaneous poles great circle angular velocity
Xiliang ZHANG, Zhiying CUI, Jianhan ZHANG, Fengwu BAI, Zhifeng WANG
《能源前沿(英文)》 2019年 第13卷 第3期 页码 590-596 doi: 10.1007/s11708-018-0602-y
关键词: secondary parabolic trough solar concentrator ray tracing method linear focus concentration ratio optical performance
Relationship between the number of tapping-induced secondary laticifer lines and rubber yield among
Yueyi CHEN,Xinsheng GAO,Xiaofei ZHANG,Weimin TIAN
《农业科学与工程前沿(英文)》 2016年 第3卷 第4期 页码 363-367 doi: 10.15302/J-FASE-2016122
关键词: Hevea brasiliensis rubber yield breeding early evaluation tapping secondary laticifer differentiation
A method for quantifying bias in modeled concentrations and source impacts for secondary particulate
Cesunica E. Ivey, Heather A. Holmes, Yongtao Hu, James A. Mulholland, Armistead G. Russell
《环境科学与工程前沿(英文)》 2016年 第10卷 第5期 doi: 10.1007/s11783-016-0866-6
关键词: Particulate matter Source apportionment Secondary particulate matter Chemical transport modeling Receptor modeling
Effects of two transition metal sulfate salts on secondary organic aerosol formation in toluene/NO
Biwu CHU, Jiming HAO, Junhua LI, Hideto TAKEKAWA, Kun WANG, Jingkun JIANG
《环境科学与工程前沿(英文)》 2013年 第7卷 第1期 页码 1-9 doi: 10.1007/s11783-012-0476-x
关键词: manganese sulfate zinc sulfate seed aerosols toluene photooxidation secondary organic aerosol
DNA组装效率的无偏差快速检验——基于qPCR而不依赖于转化的方法 Article
马晓焉, 梁昕鑫, 霍毅欣
《工程(英文)》 2019年 第5卷 第4期 页码 803-810 doi: 10.1016/j.eng.2019.06.002
合成生物学正朝着大规模、复杂化的方向发展,这在很大程度上依赖于基因模块的高效组装。传统评估DNA组装效率(AE)的方法需要进行转化,整个过程耗时长达10 h,而且容易受到各种因素的干扰。为快速、可靠地测定组装效率,本研究建立了一种基于qPCR的不依赖于转化的测定方法,用连接上的片段占初始加入片段的比例表征组装效率,3 h即可完成测定。利用该方法测定了酶切连接、Golden Gate组装以及Gibson组装法的双片段或多片段组装效率,所得结果与菌落计数法表征的组装效率呈显著正相关。该方法消除了转化过程的随机性,降低了测定偏差,优于传统的菌落计数法。随后,用此方法研究了DNA片段末端的二级结构对组装效率的影响。结果显示,所有依赖于末端序列互补的组装技术,其组装效率主要受重叠序列整体性质的影响,而发夹结构可显著降低组装效率。这种基于qPCR的测定方法将促进DNA组装技术的发展,并有助于对组装效率影响因素的评估。
标题 作者 时间 类型 操作
Functional characterization of a thermostable methionine adenosyltransferase from
Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan
期刊论文
Mechanisms for simultaneous ozonation of sulfamethoxazole and natural organic matters in secondary effluent
期刊论文
Two-echelon Emergency Response Problem and Simulation Considering Secondary Disasters
Han-peng Zhang,Yi Liao,Hui-xia Luo
期刊论文
PCDD/Fs emission, risk characterization, and reduction in China’s secondary copper production industry
Haiqian LI, Yonglong LU, Li LI
期刊论文
Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua
null
期刊论文
Simple model of sludge thickening process in secondary settlers
Yuankai ZHANG,Hongchen WANG,Lu QI,Guohua LIU,Zhijiang HE,Songzhu JIANG
期刊论文
On the location of the secondary instantaneous poles in two-degree-of-freedom spherical mechanisms
Soheil ZARKANDI
期刊论文
Optical performance analysis of an innovative linear focus secondary trough solar concentrating system
Xiliang ZHANG, Zhiying CUI, Jianhan ZHANG, Fengwu BAI, Zhifeng WANG
期刊论文
Relationship between the number of tapping-induced secondary laticifer lines and rubber yield among
Yueyi CHEN,Xinsheng GAO,Xiaofei ZHANG,Weimin TIAN
期刊论文
A method for quantifying bias in modeled concentrations and source impacts for secondary particulate
Cesunica E. Ivey, Heather A. Holmes, Yongtao Hu, James A. Mulholland, Armistead G. Russell
期刊论文
Effects of two transition metal sulfate salts on secondary organic aerosol formation in toluene/NO
Biwu CHU, Jiming HAO, Junhua LI, Hideto TAKEKAWA, Kun WANG, Jingkun JIANG
期刊论文